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美国USP35文档求助，道客巴巴上的，有链接，谁帮忙下载下
淘宝里面搜索 药典论坛 没用过，应该付点钱就可以了的吧， 怎么发给你，不会上传PDF : Originally posted by zhangran7030 at
怎么发给你，不会上传PDF 邮箱,十分感谢 BRIEFING&&
Powdered Andrographis. A new USP Dietary Supplement monograph is proposed—
See briefing under Andrographis published elsewhere in this issue of PF.
(DSB: M. Sharaf.)
RTS—C66624&&
Add the following:&&
Powdered Andrographis
DEFINITION&&
Powdered Andrographis is Andrographis reduced to a fine or very fine powder.&&
IDENTIFICATION&&
•&&A. THIN-LAYER CHROMATOGRAPHIC IDENTIFICATION TEST&&201&&
Standard solution 1:&&Use Standard solution A, prepared as directed in the test for
Content of Diterpene Lactones.&&
Standard solution 2:&&Sonicate an amount of USP Powdered Andrographis Extract
RS, equivalent to about 15 mg of diterpene lactones, for 10–15 min in 25 mL of
methanol, centrifuge, and use the supernatant.&&
Sample solution:&&Use Sample stock solution, prepared as directed in the test for
Content of Diterpene Lactones.&&
Adsorbent:&&Chromatographic silica gel mixture with an average particle size of 10–15
&m (TLC plates)&&
Application volume:&&10 &L, as 5–10 mm bands&&
Developing solvent system:&&Chloroform, acetone, and toluene (2:2:1)&&
Spray reagent:&&A mixture of 1% vanillin in alcohol and 10% sulfuric acid in alcohol
Analysis&&
Samples:&&Standard solution 1, Standard solution 2, and Sample solution&&
Use a saturated chamber. Develop the chromatograms until the solvent front has
moved about 90% of the length of the plate. Remove the plate from the chamber,
dry, spray with the Spray reagent, heat for 5–10 min at 100 , and examine under
visible light.&&
Acceptance criteria:&&The Sample solution exhibits three main grayish blue zones
with RF values of approximately 0.4, 0.6, and 0.8 that correspond in position and
color to the main zones of Standard solution 2. Standard solution 1 exhibits a grayish
blue zone due to andrographolide at an RF of about 0.4. The Sample solution
exhibits a zone similar in color and RF value to that due to andrographolide in
Standard solution 1.&&
•&&B. The retention time of the main peak of the Sample solution obtained in the test for
Content of Diterpene Lactones corresponds to that of andrographolide in Standard
solution A. Identify other diterpene lactone peaks in the Sample solution by
comparison with Standard solution B and the reference chromatogram provided with
the lot of USP Powdered Andrographis Extract RS. The Sample solution shows
additional peaks corresponding to neoandrographolide, 14-deoxy-11,12-
didehydroandrographolide, and andrograpanin.&&
COMPOSITION&&
•&&CONTENT OF DITERPENE LACTONES
Solution A:&&Dissolve 0.14 g of potassium dihydrogen phosphate in 900 mL of water,
add 0.5 mL of phosphoric acid, dilute with water to 1000 mL, mix, filter, and degas.&&
Solution B:&&Use filtered and degassed acetonitrile.&&
Standard solution A:&&Dissolve a weighed quantity of USP Andrographolide RS in
methanol to obtain a solution having a known concentration of about 1.0 mg/mL.
Transfer 5.0 mL of this solution to a 10-mL volumetric flask, dilute with acetonitrile to
volume, and mix.&&
Standard solution B:&&Transfer an amount of USP Powdered Andrographis Extract
RS, equivalent to about 25 mg of diterpene lactones, to a 50-mL volumetric flask,
add 25 mL of methanol, heat gently for 15–20 min, dilute with acetonitrile to volume,
and mix. Before injection, pass through a membrane filter having a 0.45-&m or finer
porosity, discarding the first 5 mL of the filtrate.
Sample stock solution:&&Transfer about 2.0 g of Powdered Andrographis to a 250-mL
flask fitted with a reflux condenser. Add 50 mL of methanol, reflux on a water bath for
15 min, cool to room temperature, and decant the supernatant. Repeat until the last
extract is colorless. Combine the extracts, filter, concentrate under vacuum, and
adjust the volume to 50.0 mL using methanol. Transfer 25.0 mL of this solution to a
50-mL volumetric flask, dilute with acetonitrile to volume, and mix. Before injection,
pass through a membrane filter having a 0.45-&m or finer porosity, discarding the
first 5 mL of the filtrate.&&
Sample solution:&&Transfer 25.0 mL of Sample stock solution to a 50-mL volumetric
flask, dilute with acetonitrile to volume, and mix. Before injection, pass through a
membrane filter having a 0.45-&m or finer porosity, discarding the first 5 mL of the
filtrate.&&
Mobile phase:&&See the gradient table below.
Time (min)& &Solution A (%)& & Solution B (%)
0& && && && &95& && && && &&&5
18& && && &&&55& && && && & 45
25& && && &&&20& && && && & 80
28& && && &&&20& && && && & 80
35& && && &&&55& && && && & 45
40& && && &&&95& && && && &&&5
45& && && &&&95& && && && &&&5
Chromatographic system& &
(See Chromatography&&621&&, System Suitability.)
Mode:&&LC&&
Detector:&&UV 223 nm&&
Column:&&4.6-mm × 25- 5-&m packing L1&&
Flow rate:&&1.5 mL/min&&
Injection size:&&20 &L&&
System suitability&&
Samples:&&Standard solution A and Standard solution B&&
Suitability requirements&&
Column efficiency:&&NLT 5000 theoretical plates, Standard solution A&&
Tailing factor:&&NMT 1.5 for the andrographolide peak, Standard solution A&&
Relative standard deviation:&&NMT 2.0%, determined from the andrographolide
peak for replicate injections, Standard solution A&&
Resolution:&&NLT 5 between neoandrographolide and 14-deoxy-11,12-
didehydroandrographolide peaks, Standard solution B&&
Analysis&&
Samples:&&Standard solution A, Standard solution B, and Sample solution&&
Using the chromatogram of Standard solution A, Standard solution B, and the
reference chromatogram provided with the lot of USP Powdered Andrographis
Extract RS, identify the retention times of the peaks corresponding to the different
diterpene lactones. The approximate relative retention times of the different
diterpene lactones are provided in the following table:
Analyte& && && && && && && && && & Relative Retention Time
Andrographolide& && && && && && && && && && && && & 1.00
Neoandrographolide& && && && && && && && && && && & 1.16
14-Deoxy-11,12-didehydroandrographolide& && && && && & 1.31
Andrograpanin& && && && && && && && && && && && && &1.50
Separately calculate the percentages of andrographolide, neoandrographolide, 14-
deoxy-11,12-didehydroandrographolide, and andrograpanin in the portion of
Powdered Andrographis taken:&&
Result = 10(CS /W)(rU /rS ) × F
rU&&= peak response for each diterpene lactone from the Sample solution
rS&&= peak response for andrographolide from Standard solution A
CS = concentration of USP Andrographolide RS in the Standard solution A
W = weight of Powdered Andrographis taken to prepare the Sample solution (g)
F = conversion factor for each analyte (1.00 for andrographolide, 3.90 for
neoandrographolide, 1.45 for 14-deoxy-11,12-didehydroandrographolide, and
2.65 for andrograpanin)
Acceptance criteria:&&NLT 1.0%, on the dried basis, of the sum of the percentages of
andrographolide, neoandrographolide, 14-deoxy-11,12-didehydroandrographolide,
and andrograpanin&&
IMPURITIES&&
Inorganic Impurities&&
•& &ARTICLES OF BOTANICAL ORIGIN, Acid-Insoluble Ash&&561&&: NMT 3.0%&&
•& &HEAVY METALS, Method II& &231&&: NMT 20 ppm&&
Organic Impurities&&
•&&PROCEDURE: ARTICLES OF BOTANICAL ORIGIN, General Method for Pesticide Residues
Analysis&&561&&: Meets the requirements&&
SPECIFIC TESTS&&
•&&BOTANIC CHARACTERISTICS
Macroscopic:&&It is a grayish brown powder.&&
Histology&&
Microscopic examination:&&It reveals cells of the upper and lower epidermis of the
leaves, some cells containing large cystoliths, up to 36 &m in diameter and 180 &m
long, with a hilum-shaped s 1–4 celle
disk-shaped glandular hairs, 8-celled head
diacytic stomata
mostly on stem epidermal cells, some cells containing
cystoliths, stomata, nonglandular hairs and glandular hairs similar to those of the
thin-wal ac
vessels, with spiral and scalariform thickening.&&
•& &LOSS ON DRYING&&731&&: Dry 1.0 g of finely Powdered Andrographis at 105&&for 3 h: it loses NMT 12.0% of its weight.&&
•& &ARTICLES OF BOTANICAL ORIGIN, Total Ash&&561&&: NMT 15%, determined on 1.0 g of finely Powdered Andrographis&&
•& &ARTICLES OF BOTANICAL ORIGIN, Alcohol-Soluble Extractives, Method 2&&561&&: NLT 8.0%&&
•& &MICROBIAL ENUMERATION TESTS—NUTRITIONAL AND DIETARY SUPPLEMENTS&&2021&&: The total aerobic bacterial count does not exceed 105 cfu/g; the total combined moldsand yeasts count does not exceed 103 cfu/g; and the bile-tolerant Gram-negative bacteria does not exceed 103 cfu/g.
ABSENCE OF SPECIFIED MICROORGANISMS—NUTRITIONAL AND DIETARY SUPPLEMENTS 2022&&: Meets the requirements of the tests for absence of Salmonella species and Escherichia coli&&
ADDITIONAL REQUIREMENTS&&
•&&PACKAGING AND STORAGE: Preserve in well-closed containers, protected from light andmoisture, and store at room temperature.&&
•&&LABELING: The label states the Latin binomial and, following the official name, the parts of the plant contained in the article.&&
•& &USP REFERENCE STANDARDS&&11& &
USP Andrographolide RS&&
USP Powdered Andrographis Extract RS&&2S (USP33) : Originally posted by zhangran7030 at
已发送 非常感谢 : Originally posted by zhangran7030 at
BRIEFING&&
Powdered Andrographis. A new USP Dietary Supplement monograph is proposed—
See briefing under Andrographis published elsewhere in this issue of PF.
(DSB: M. Sharaf.)
RTS—C66624&&
... 实在抱歉，不知怎么弄得，想给你20个金币的，结果才给了一个，再追加补上了啊，怎么才能给你再评分啊，真是羞愧啊 : Originally posted by zhangran7030 at
BRIEFING&&
Powdered Andrographis. A new USP Dietary Supplement monograph is proposed—
See briefing under Andrographis published elsewhere in this issue of PF.
(DSB: M. Sharaf.)
RTS—C66624&&
... 先送一朵小红花吧，实在抱歉了:cry:&& 查看话题
怎么下载一篇道客巴巴的文章，要100积分的？求助？！！！！
文章题目：
国泰君安-100406-金利华电（300069）国内高压线路玻璃绝缘子领导者
请求啊！！！木虫神人比较多，不要让我失望啊！
/p-.html 我传到网盘里了，下载地址：/urllink.php?id=m8cb3lcsa7j76 : Originally posted by xinmor at
我传到网盘里了，下载地址：/urllink.php?id=m8cb3lcsa7j76 :hand:谢谢你啊，不过我想知道到底要怎么才能下载呢？谢谢啦，授人以鱼不如授人以渔！ : Originally posted by airstone1062 at
:hand:谢谢你啊，不过我想知道到底要怎么才能下载呢？谢谢啦，授人以鱼不如授人以渔！ 道客巴巴没有什么特别好的办法的，论坛上有人说过用截屏软件来。
我是在其他地方找到的这个文章。 能在线直接看见文章全文，说明这篇文章已经下载到你的电脑缓存区了。有一个办法可以试一试，先清空IE临时文件夹里面所有的文件，然后刷新当前这个页面，然后在回去看IE的临时文件夹，里面应该就有这篇文章了！ : Originally posted by sunupper at
能在线直接看见文章全文，说明这篇文章已经下载到你的电脑缓存区了。有一个办法可以试一试，先清空IE临时文件夹里面所有的文件，然后刷新当前这个页面，然后在回去看IE的临时文件夹，里面应该就有这篇文章了！ :hand::tiger28::tiger05:谁可以帮我在道客巴巴上面下载一个文档！急求、、、_百度知道
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